An Electrochemical Biosensor Array Platform for the Continuous, Dual-technique Monitoring of Multiple Biomarkers in Organ-On-Chips
| dc.contributor.committeeChair | Cliffel, David E | |
| dc.creator | Tuladhar, Pragun Ratna | |
| dc.creator.orcid | 0000-0002-0808-1234 | |
| dc.date.accessioned | 2025-09-26T11:08:47Z | |
| dc.date.created | 2025-08 | |
| dc.date.issued | 2025-07-15 | |
| dc.date.submitted | August 2025 | |
| dc.description.abstract | Electrochemical biosensors are ideal complementary tools to evaluate organ-on-chip (OoC) systems because they can be miniaturized, allow multiplexed measurements, have rapid response times, and require minimal sample preparation while being inexpensive. In this work, a microfluidic multianalyte electrochemical sensor array (µMESA) platform is presented for the continuous, dual-technique, multianalyte detection of biomarkers in OoCs. Surface and electrochemical characterization of the screen-printed electrode (SPE) revealed heterogeneous Pt surfaces that were stable and reproducible enough for sensing. The SPE with glucose, lactate, glutamate, and acetylcholine biosensors was integrated into the µMESA and simultaneous, selective detection of the four corresponding biomarkers under laminar flow was demonstrated. The µMESA was used to measure glucose consumption by bacterial cells. Osmium-based redox polymers (OsPVIAA) with various metal-loadings were synthesized, but higher loadings only correlated to larger oxidation peaks up to 32%. OsPVIAA was highly successful in preventing background interfering signals from cell media. Optimizing hydrogel crosslinking and incorporating a thin poly(3,4-ethylenedioxythiophene) (PEDOT) film produced an OsPVIAA-glucose sensor that minimized loss of the polymer to the bulk solution. Fluorosilicone-based ion-selective membranes for K⁺ and Ca²⁺ were also prepared on the SPE. However, they exhibited super-Nernstian responses during calibrations. Simultaneous amperometric (acetylcholine, glutamate) and potentiometric detection (K⁺, Ca²⁺) of analytes was also presented. This work explored the multianalyte detection, interference mitigation, and sensor stability aspects of electrochemical biosensing. By demonstrating its applicability in dynamic environments like flow conditions and biological media, the µMESA platform was shown to be well-suited to electrochemically analyze tissue functions in OoCs. | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.uri | https://hdl.handle.net/1803/19885 | |
| dc.language.iso | en | |
| dc.subject | biosensors | |
| dc.subject | electrochemistry | |
| dc.title | An Electrochemical Biosensor Array Platform for the Continuous, Dual-technique Monitoring of Multiple Biomarkers in Organ-On-Chips | |
| dc.type | Thesis | |
| dc.type.material | text | |
| local.embargo.lift | 2027-08-01 | |
| local.embargo.terms | 2027-08-01 | |
| thesis.degree.discipline | Chemistry | |
| thesis.degree.grantor | Vanderbilt University Graduate School | |
| thesis.degree.level | Doctoral | |
| thesis.degree.name | PhD |
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