Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Randomized Controlled Trial
. 2024 Aug;26(8):3458-3461.
doi: 10.1111/dom.15628. Epub 2024 Apr 30.

Human adipose tissue expression of uncoupling protein 1 in response to intravenous administration of B-type natriuretic peptide hormone: Results from a randomized controlled crossover study

Katherine N Bachmann  1   2   3 , Ryan P Ceddia  4 , Deepak K Gupta  3   4 , Sheila Collins  4   5 , Thomas J Wang  6
Affiliations
Randomized Controlled Trial

Human adipose tissue expression of uncoupling protein 1 in response to intravenous administration of B-type natriuretic peptide hormone: Results from a randomized controlled crossover study

Katherine N Bachmann et al. Diabetes Obes Metab. 2024 Aug.
No abstract available

PubMed Disclaimer

Conflict of interest statement

CONFLICT OF INTEREST STATEMENT

Katherine N. Bachmann is currently an employee at Amgen, but her work for this project was conducted in the past when she had been an employee at Vanderbilt and Veterans Administration, and is not related to her work at Amgen. Amgen played no role in the study design, conduct, funding, or preparation of this manuscript.

Figures

FIGURE 1
FIGURE 1
B-type natriuretic peptide1–32 (BNP) infusion in humans promotes lipolysis and increased uncoupling protein 1 (UCP1) gene expression in subcutaneous adipose tissue. (A) The ratio of free fatty acid levels at 60 min divided by baseline was calculated under each condition (BNP infusion and placebo infusion). BNP infusion led to within-participant increases in circulating free fatty acids relative to placebo infusion (p = 0.038 for fold-change of BNP/placebo). (B) UCP1 expression was quantified by quantitative real-time reverse-transcription polymerase chain reaction and normalized to a housekeeping gene. Nine adipose tissue samples across five participants are depicted. Each symbol corresponds to a specific participant, with a line connecting the paired tissue samples. All four participants with paired tissue samples experienced an increase in adipose tissue UCP1 expression after BNP treatment relative to placebo (median within-participant fold-change in UCP1 expression for BNP infusion relative to placebo = 3.0, 25th–75th percentile 2.5–4.0; p = 0.034 for fold-change of BNP/placebo). (C) Summary figure of the signalling pathway stimulated by BNP in adipocytes. BNP is expected to increase intracellular cyclic guanosine monophosphate (cGMP) concentrations, which activates protein kinase G (PKG). PKG is known to activate distinct yet overlapping signalling pathways leading to increased lipolysis and UCP1 transcription. Created with BioRender.com. For all figures, box-and-whisker plots depict median, 25th and 75th percentile, minimum, and maximum. Each participant is represented by a unique symbol, which remains consistent through the figure panels. Symbols representing obese participants are depicted in red, while symbols for lean participants are depicted in blue. GTP, guanosine triphosphate; NPRA, natriuretic peptide receptor A.
See this image and copyright information in PMC

References

    1. Bordicchia M, Liu D, Amri EZ, et al. Cardiac natriuretic peptides act via p38 MAPK to induce the brown fat thermogenic program in mouse and human adipocytes. J Clin Invest. 2012;122(3):1022–1036. - PMC - PubMed
    1. Miyashita K, Itoh H, Tsujimoto H, et al. Natriuretic peptides/cGMP/cGMP-dependent protein kinase cascades promote muscle mitochondrial biogenesis and prevent obesity. Diabetes. 2009;58(12):2880–2892. - PMC - PubMed
    1. Cannone V, Boerrigter G, Cataliotti A, et al. A genetic variant of the atrial natriuretic peptide gene is associated with cardiometabolic protection in the general community. J Am Coll Cardiol. 2011;58(6):629–636. - PMC - PubMed
    1. Newton-Cheh C, Larson MG, Vasan RS, et al. Association of common variants in NPPA and NPPB with circulating natriuretic peptides and blood pressure. Nat Genet. 2009;41(3):348–353. - PMC - PubMed
    1. Sengenes C, Stich V, Berlan M, et al. Increased lipolysis in adipose tissue and lipid mobilization to natriuretic peptides during low-calorie diet in obese women. Int J Obes Relat Metab Disord. 2002;26(1):24–32. - PubMed

Substances